Location: Biosciences Room 138

The Apotome is a widefield fluorescence microscope which allows you to create optical sections of your samples free of scattered light. With optical sectioning structured illumination (OS-SIM) only the focal plane appears in your final image. The Apotome projects a grid structure onto the focal plane of your specimen, then moves it into three positions using a scanning mechanism acquiring an image at each position. The system processes the three images into one optical section with improved contrast and increased resolution. The process occurs automatically making this as easy to use as an standard widefield fluorescence microscope.