Staff profile
Overview
Affiliation |
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Associate Professor in the Department of Biosciences |
Visiting Associate Professor in the Department of Chemistry |
Research interests
- bacteriology
- bioinorganic chemistry
- biotechnology
- metalloproteins
- microbiology
- nickel
- protein chemistry
- protein engineering
Publications
Chapter in book
Journal Article
- Chivers, P. T., Basak, P., & Maroney, M. J. (2024). One His, two His…the emerging roles of histidine in cellular nickel trafficking. Journal of Inorganic Biochemistry, 259, Article 112668. https://doi.org/10.1016/j.jinorgbio.2024.112668
- Basak, P., Cabelli, D. E., Chivers, P. T., Farquhar, E. R., & Maroney, M. J. (2023). In vitro maturation of NiSOD reveals a role for cytoplasmic histidine in processing and metalation. Metallomics, 15(11), Article mfad054. https://doi.org/10.1093/mtomcs/mfad054
- Ni, J., Wood, J. L., White, M. Y., Lihi, N., Markham, T. E., Wang, J., Chivers, P. T., & Codd, R. (2023). Reduction-cleavable desferrioxamine B pulldown system enriches Ni( ii )-superoxide dismutase from a Streptomyces proteome. RSC Chemical Biology, 4, Article 1064. https://doi.org/10.1039/d3cb00097d
- Foster, A. W., Young, T. R., Chivers, P. T., & Robinson, N. J. (2022). Protein metalation in biology. Current Opinion in Chemical Biology, 66, Article 102095. https://doi.org/10.1016/j.cbpa.2021.102095
- Osman, D., Martini, M. A., Foster, A. W., Chen, J., Scott, A. J., Morton, R. J., Steed, J. W., Lurie-Luke, E., Huggins, T. G., Lawrence, A. D., Deery, E., Warren, M. J., Chivers, P. T., & Robinson, N. J. (2019). Bacterial sensors define intracellular free energies for correct enzyme metalation. Nature Chemical Biology, 15(3), 241-249. https://doi.org/10.1038/s41589-018-0211-4
- Huang, H.-T., Bobst, C., Iwig, J., Chivers, P., Kaltashov, I., & Maroney, M. (2018). Co(II) and Ni(II) binding of the Escherichia coli transcriptional repressor RcnR orders its N-terminus, alters helix dynamics, and reduces DNA affinity. Journal of Biological Chemistry, 293(1), 324-332. https://doi.org/10.1074/jbc.ra117.000398
- Carr, C., Musiani, F., Huang, H.-T., Chivers, P., Ciurli, S., & Maroney, M. (2017). Glutamate Ligation in the Ni(II)- and Co(II)-Responsive Escherichia coli Transcriptional Regulator, RcnR. Inorganic Chemistry, 56(11), 6459-6476. https://doi.org/10.1021/acs.inorgchem.7b00527
- Foster, A., Pernil, R., Patterson, C., Scott, A., Pålsson, L.-O., Pal, R., Cummins, I., Chivers, P., Pohl, E., & Robinson, N. (2017). A tight tunable range for Ni(II) sensing and buffering in cells. Nature Chemical Biology, 13(4), 409-414. https://doi.org/10.1038/nchembio.2310
- Moore, S., Sowa, S., Schuchardt, C., Deery, E., Lawrence, A., Ramos, J., Billig, S., Birkemeyer, C., Chivers, P., Howard, J., Rigby, S., Layer, G., & Warren, M. (2017). Elucidation of the biosynthesis of the methane catalyst coenzyme F430. Nature, 543(7643), 78-82. https://doi.org/10.1038/nature21427
- Denby, K., Iwig, J., Bisson, C., Westwood, J., Rolfe, M., Sedelnikova, S., Higgins, K., Maroney, M., Baker, P., Chivers, P., & Green, J. (2016). The mechanism of a formaldehyde-sensing transcriptional regulator. Scientific Reports, 6, Article 38879. https://doi.org/10.1038/srep38879
- Chivers, P. (2015). Nickel recognition by bacterial importer proteins. Metallomics, 7(4), 590-595. https://doi.org/10.1039/c4mt00310a
- Higgins, K., Hu, H., Chivers, P., & Maroney, M. (2013). The Effects of Select Histidine to Cysteine Mutations on Transcriptional Regulation by E. coli RcnR. Biochemistry, 52(1), 84-97. https://doi.org/10.1021/bi300886q
- Higgins, K., Chivers, P., & Maroney, M. (2012). Role of the N-terminus in determining metal-specific responses in the E. coli Ni- and Co-responsive metalloregulator, RcnR. Journal of the American Chemical Society, 134(16), 7081-7093. https://doi.org/10.1021/ja300834b
- Chivers, P., Benanti, E., Heil-Chapdelaine, V., Iwig, J., & Rowe, J. (2012). Identification of Ni-(L-His)2 as a substrate for NikABCDE-dependent nickel uptake in Escherichia coli. Metallomics, 4(10), 1043 -1050. https://doi.org/10.1039/c2mt20139a
- Benanti, E., & Chivers, P. (2011). Helicobacter pylori NikR Protein Exhibits Distinct Conformations When Bound to Different Promoters. Journal of Biological Chemistry, 286(18), 15728-15737. https://doi.org/10.1074/jbc.m110.196055
- Choi, S., Chivers, P., & Berg, D. (2011). Point mutations in Helicobacter pylori's fur regulatory gene that alter resistance to metronidazole, a prodrug activated by chemical reduction. PLoS ONE, 6(3), https://doi.org/10.1371/journal.pone.0018236
- Benanti, E., & Chivers, P. (2010). Geobacter uraniireducens NikR displays a DNA binding mode distinct from other members of the NikR family. Journal of Bacteriology, 192(17), 4327-4336. https://doi.org/10.1128/jb.00152-10
- Iwig, J., & Chivers, P. (2010). Coordinating intracellular nickel – metal-site structure-function relationships and the NikR and RcnR repressors. Natural Product Reports, 27(5), 658-667. https://doi.org/10.1039/b906683g
- Herbst, R., Perovic, I., O'Brien, K., Chivers, P., Pochapsky, S., Pochapsky, T., & Maroney, M. (2010). The communication between the zinc and nickel sites in dimeric HypA: Metal recognition and pH sensing. Journal of the American Chemical Society, 132(30), https://doi.org/10.1021/ja1005724
- Iwig, J., & Chivers, P. (2009). DNA Recognition and Wrapping by Escherichia coli RcnR. Journal of Molecular Biology, 393(2), https://doi.org/10.1016/j.jmb.2009.08.038
- Benanti, E., & Chivers, P. (2009). An intact urease assembly pathway is required to compete with NikR for nickel ions in Helicobacter pylori. Journal of Bacteriology, 191(7), 2405-2408. https://doi.org/10.1128/jb.01657-08
- Bradley, M., Chivers, P., & Baker, N. (2008). Molecular dynamics simulation of the Escherichia coli NikR protein: equilibrium conformational fluctuations reveal interdomain allosteric communication pathways. Journal of Molecular Biology, 378(5), 1155-1173. https://doi.org/10.1016/j.jmb.2008.03.010
- Iwig, J., Leitch, S., Herbst, R., Maroney, M., & Chivers, P. (2008). Ni(II) and Co(II) sensing by Escherichia coli RcnR. Journal of the American Chemical Society, 130(24), https://doi.org/10.1021/ja710067d
- Leitch, S., Bradley, M., Rowe, J., Chivers, P., & Maroney, M. (2007). Nickel-specific response in the transcriptional regulator, Escherichia coli NikR. Journal of the American Chemical Society, 129(16), 5085-5095. https://doi.org/10.1021/ja068505y
- Benanti, E., & Chivers, P. (2007). The N-terminal arm of the Helicobacter pylori Ni2+-dependent transcription factor NikR is required for specific DNA binding. Journal of Biological Chemistry, 282(28), https://doi.org/10.1074/jbc.m702982200
- Chivers, P. (2007). A Galvanizing Story – Protein Stability and Zinc Homeostasis. Journal of Bacteriology, 189(8), 2953-2954. https://doi.org/10.1128/jb.00173-07
- Kennedy, D., Herbst, R., Iwig, J., Chivers, P., & Maroney, M. (2007). A Dynamic Zn Site in Helicobacter pylori HypA: A Potential Mechanism for Metal-Specific Protein Activity. Journal of the American Chemical Society, 129(1), https://doi.org/10.1021/ja066958x
- Iwig, J., Rowe, J., & Chivers, P. (2006). Nickel homeostasis in Escherichia coli – the rcnR-rcnA efflux pathway and its linkage to NikR function. Molecular Microbiology, 62(1), https://doi.org/10.1111/j.1365-2958.2006.05369.x
- Rowe, J., Starnes, G., & Chivers, P. (2005). Complex transcriptional control links NikABCDE-dependent nickel transport with hydrogenase expression in Escherichia coli. Journal of Bacteriology, 187(18), 6317-6323. https://doi.org/10.1128/jb.187.18.6317-6323.2005
Supervision students
Archie Howell
Research Postgraduate (PhD)
Ayuki Shimpo
Research Postgraduate (PhD)
Caitlin Gould
Research Postgraduate (MSc)
Emily Wallace
Research Postgraduate (MSc)
Grace Pantasri
Research Postgraduate (PhD)
Rosella Pinckney
Research Postgraduate (PhD)
Safa Chougule
Research Postgraduate (PhD)